Techno-Path Distribution Ltd.
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电话353 61 335844
传真353 61 203034
>电子邮件des.joyce@techno-path.com
网站www.techno-path.com
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产品MicroStationOdinOmniLog版权所有 2023 Biolog，保留所有权利用户隐私政策Site by Metropolis
产品产品实验室服务应用资源网络研讨会新闻与活动贸易展览新闻发布公司关于我们联系方式提交样品全球经销商约旦、黎巴嫩、土耳其、伊拉克和巴勒斯坦领土AGBL Levant（区域办事处）
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国家/地区阿富汗阿尔及利亚安哥拉亚美尼亚澳大利亚奥地利阿塞拜疆巴林孟加拉国比荷卢经济联盟贝宁博茨瓦纳巴西保加利亚布基纳法索布隆迪喀麦隆加拿大乍得智利中国哥伦比亚刚果克罗地亚塞浦路斯捷克共和国刚果丹麦吉布提埃及赤道几内亚爱沙尼亚埃塞俄比亚芬兰法国加蓬冈比亚德国加纳希腊几内亚几内亚比绍香港匈牙利印度印度尼西亚伊拉克爱尔兰以色列意大利象牙海岸日本约旦哈萨克斯坦肯尼亚韩国科威特吉尔吉斯斯坦拉脱维亚黎巴嫩莱索托利比亚立陶宛马达加斯加马拉维马来语西亚马里马耳他毛里求斯毛里求斯墨西哥摩洛哥莫桑比克纳米比亚尼泊尔新西兰尼日尔尼日利亚挪威阿曼巴基斯坦巴勒斯坦秘鲁菲律宾波兰波尔图加尔波多黎各卡塔尔俄罗斯卢旺达沙特阿拉伯塞内加尔塞拉利昂利比里亚新加坡斯洛文尼亚西班牙斯里兰卡斯威士兰瑞典瑞士台湾塔吉克斯坦坦桑尼亚多哥突尼斯土耳其土库曼斯坦阿联酋乌克兰英国美国乌兹别克斯坦也门赞比亚津巴布韦产品请选择产品：
产品MicroStationOdinOmni日志版权所有 2023 Biolog，保留所有权利用户隐私政策Site by Metropolis
产品产品实验室服务应用资源网络研讨会新闻与活动贸易展览新闻发布公司关于我们联系方式提交样品产品货号：BM0627
产品价格：880元/100ul1680元/200ul6800元/1ml
检验物种：human, mouse, rat, pig, monkey, rabbit, chicken
应用范围：WB, IHC-P
产品类型：Monoclonal (Clone: AC-15)
发货周期：现货
选择规格:
880元/100ul 1680元/200ul 6800元/1mlslightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.
产品应用细节
为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
Western blot:1:2000-20000Immunohistochemistry in paraffin section IHC-(P):1:50-400(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.
Figure 1. Western blot analysis of anti- β-Actin antibody (BM0627).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Human HELA whole cell lysates,Lane 2: Human HepG2 whole cell lysates,Lane 3: Monkey kidney tissue lysates,Lane 4: Monkey liver tissue lysates,Lane 5: Rat brain tissue lysates,Lane 6: Rat PC-12 whole cell lysates,Lane 7: Mouse brain tissue lysates,Lane 8: Mouse ANA-1 whole cell lysates,Lane 9: Rabbit intestines tissue lysates,Lane 10: Rabbit intestines tissue lysates,Lane 11: Pig intestines tissue lysates.Use rabbit anti- β-Actin 1:5000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for β-Actin at approximately 42KD. The expected band size for β-Actin is at 42KD.
Figure 2.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Mouse Intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 3.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Rat Intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 4.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Human Intestinal Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 5.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Human Lung Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 6.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Human Mammary Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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